Pichia Protocols by James M. Cregg

Pichia Protocols



Download Pichia Protocols




Pichia Protocols James M. Cregg
Language: English
Page: 260
Format: pdf
ISBN: 1588294293, 9781588294296
Publisher: Humana Press

This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.

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